Ke mefuta efe ea mekhoa ea spermine synthesis? Metsoako ea sehlooho ke efe?

Spermidine ke polyamine ea bohlokoa e teng ka bongata linthong tse phelang 'me e kenya letsoho lits'ebetsong tse fapaneng tsa likokoana-hloko tse kang ho ata ha lisele, ho arola le apoptosis. Haholo-holo ho na le mefuta e mengata ea mekhoa ea spermine synthesis: biosynthesis, lik'hemik'hale tsa lik'hemik'hale le enzymatic synthesis. Mokhoa o mong le o mong o na le melemo le likotsi tsa ona tse ikhethang le maemo a ts'ebeliso.

Biosynthesis ke tsela e ka sehloohong ea motsoako oa spermine, oo hangata o etsoang ka letoto la karabelo ea enzymatic liseleng. The biosynthesis ea spermine haholo-holo e itšetlehile ka metabolism ea amino acid, haholo-holo lysine le arginine. Ntlha ea pele, lysine e fetoloa aminobutyric acid (Putrescine) ke lysine decarboxylase, ebe acid e aminobutyric e kopana le amino acid tlas'a ketso ea spermine synthase ho qetella e theha spermine. Ho phaella moo, motsoako oa spermine o boetse o kenyelletsa metabolism ea li-polyamine tse ling, tse kang putrescine (Cadaverine) le spermine (Spermine). Liphetoho ka har'a mahloriso a li-polyamine tsena liseleng li tla ama sebopeho sa spermine.

Motsoako oa lik'hemik'hale ke mokhoa o sebelisoang hangata bakeng sa ho kopanya spermine ka laboratoring. Metsoako e bonolo ea lintho tse phelang hangata e fetoloa spermine ka liphetoho tsa lik'hemik'hale. Litsela tse tloaelehileng tsa ho kopanya lik'hemik'hale li qala ho tloha ho li-amino acid 'me qetellong li fumana peō ea botona ka letoto la esterification, phokotso le karabelo ea amination. Molemo oa mokhoa ona ke hore o ka etsoa tlas'a maemo a laoloang, bohloeki ba sehlahisoa bo phahame, 'me bo loketse lipatlisiso tse nyenyane tsa laboratori. Leha ho le joalo, motsoako oa lik'hemik'hale hangata o hloka hore ho sebelisoe metsoako ea tlhaho le li-catalyst, tse ka bang le tšusumetso e itseng tikolohong.

Enzymatic synthesis ke mokhoa o mocha oa ho kopanya o ntlafalitsoeng lilemong tsa morao tjena, o sebelisang karabelo e khethehileng ea enzyme-catalyzed ho kopanya peō ea botona. Melemo ea mokhoa ona ke maemo a bonolo a karabelo, khetho e phahameng, le botsoalle ba tikoloho. Ka theknoloji ea boenjiniere ba liphatsa tsa lefutso, spermine synthase e sebetsang e ka fumanoa, kahoo e ntlafatsa bokhoni ba ho kopanya. Enzymatic synthesis e na le menyetla e pharaletseng ea ts'ebeliso tlhahisong ea indasteri, haholo-holo mafapheng a biomedicine le li-additives tsa lijo.

Likarolo tse ka sehloohong tsa spermine ke metsoako ea polyamine, ho akarelletsa spermine, putrescine le triamine. Sebopeho sa limolek'hule tsa spermine se na le lihlopha tse ngata tsa amino le tsa imino, 'me li na le ts'ebetso e matla ea likokoana-hloko. Liphuputso li bontšitse hore spermine e na le karolo ea bohlokoa ho ata ha lisele, ho thibela oxidation le ho thibela botsofali. Lilemong tsa morao tjena, liphuputso tse ntseng li eketseha li fumane hore spermine e boetse e amana haufi-ufi le ho hlaha le nts'etsopele ea maloetse a sa tšoaneng, a kang kankere, lefu la pelo le methapo ea mali. Ka hona, ho qaptjoa le ho sebelisoa ha spermine ho hohetse tlhokomelo e atileng.

Lits'ebetsong tse sebetsang, spermine e ka sebelisoa eseng feela e le reagent bakeng sa lipatlisiso tsa baeloji, empa hape e le tlatsetso ea lijo le sehlahisoa sa sehlahisoa sa bophelo bo botle. Ha batho ba ela hloko bophelo bo botle, tlhokahalo ea 'maraka ea spermine e ntse e eketseha butle-butle. Ka ho ntlafatsa mokhoa oa ho kopanya oa spermine, lihlahisoa tsa eona le bohloeki li ka eketsoa, ​​​​'me litšenyehelo tsa tlhahiso li ka fokotsoa, ​​kahoo li khothalletsa tšebeliso ea tsona likarolong tse sa tšoaneng.

Ka kakaretso, mekhoa ea ho kopanya ea spermine haholo-holo e kenyelletsa biosynthesis, metsoako ea lik'hemik'hale le enzymatic synthesis. Mokhoa o mong le o mong o na le melemo e ikhethang le maemo a sebetsang. Lipatlisiso tsa nako e tlang li ka shebana le ho ntlafatsa ts'ebetso ea ts'ebetso, ho fokotsa tšusumetso ea tikoloho le ho holisa libaka tsa ts'ebeliso. Ka tsoelopele e tsoelang pele ea mahlale le thekenoloji, ho kopanngoa le ho sebelisoa ha spermine ho tla tlisa menyetla e mecha ea ntlafatso.